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KMID : 1005220070220010075
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Journal of Embryo Transfer
2007 Volume.22 No. 1 p.75 ~ p.80
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Activation and In Vitro Development of Porcine Oocytes Treated with Ethanol, $Ca^{2+}-Ionophore$ and Strontium
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Lee Ji-Woong
Kang Man-Jong
Moon Seung-Ju
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Abstract
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The objective of this study was to examine the optimal concentration and the exposure time of ethanol, Ca-ionophore, and strontium to achieve massive recipient oocytes in porcine. The cleavage (51.4% vs. $21.3{\sim}44.3%$) and embryo development rates (45% vs. $13.3{\sim}29.9%$) were significantly higher (p.0.05) in oocytes treated with 10% ethanol for 10 min than other treatments. The oocytes treated with 25mM Ca-ionophore for a minimum of 2min and 20mM strontium for a minimum of 6h showed significantly higher cleavage and embryo development rates than those of other treatments (P<0.05). Cleavage rate with duplicated ethanol treatment was significantly lower than those with ethanol alone (P<0.05). The cleavage rate and embryo development rates were significantly lower in duplicated strontium treatment than those in both alone and combination (P<0.05). But the cleavage and embryo development rates in treatment with Ca-ionophore were significantly higher in combined treatment (Ca-ionophore and cycloheximide) than those in single or duplicated treatment (P<0.05). These results might induce establishment of the optimal concentration and the exposure time on activation media to build up activation condition of porcine oocytes.
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KEYWORD
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activation, porcine, ethanol, Ca-ionophore, strontium
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